Intellex Acquires Expert by Big Village

We're thrilled to announce that Intellex has acquired Expert by Big Village, effective March 22, 2024. This strategic move enhances our capabilities and strengthens our commitment to delivering exceptional solutions to our customers.

Stay tuned for more updates on how this acquisition will benefit our clients and experts.

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Expert Details

Protein aggregation, characterization, purification

ID: 732518 Massachusetts, USA

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Expert leads a team of exceptionally talented downstream scientists to develop scalable downstream processes for viral vectors for use in Gene, Cell and Oncolytic therapies and for Vaccine production and for therapeutics proteins and antibodies.

Keywords: Process development, DSP, Downstream processes, Gene therapy, Oncolytic Immunotherapy, AAV, Adenovirus, Lentivirus, enveloped-virus, viral vector purification, vaccine characterization, protein characterization, analytical characterization, antibody purification, biologics, Depth filtration, TFF, Tangential flow filtration, UF/DF, Chromatography, Vaccine, Covid-19, HPLC (IEX, SEC), RP-HPLC, AKTA FPLC, TFF, dynamic light scattering (DLS), DSC, CD, AUC, protein chemistry, biochemistry, NMR spectroscopy, SAXS, protein engineering, UV-vis, fluorescence spectroscopy, gel electrophoresis, biochemical, biophysical characterization, Fluorescence biophysical characterization, data analysis

SUMMARY
• Innovative protein biophysicist and biochemist with 10+ years of proven expertise in protein biochemistry and biophysics
• Led 150 + projects and worked in collaborative and multidisciplinary teams within academic and industrial set-ups
• Performed and supervised projects involving protein and antibody purification and characterization
• Interacted with teams within and outside the company to prepare statement of work, reports, expanded business opportunities in a one-to-one or team settings
• Prepared cost-benefit analysis on an in-house project to improve efficiency and curb losses in a long run

SKILLS
• Nucleic acids: Isolation of mRNA from mouse brain, isolation of plasmid DNA from E. coli DH5 strain, reverse transcription of mRNA, Polymerase Chain Reaction (PCR), Molecular cloning
• Recombinant proteins: Expression and purification of proteins such as prion protein and its variant, αB-crystallin, α-synuclein, 2-microglobulin, CRABP1 and its variants using bacterial expression system, 15N and 13C isotope labeling of proteins, protein modification using fluorophore labeling, SDS-PAGE, Western blotting, and Blue-Native PAGE
• Protein purification: Affinity chromatography, anion and cation exchange chromatography (CEX), HIC, preparative SEC, AKTA FPLC using custom packed columns including XKs (16, 26 and 50) and BPG100 columns, and Tangential flow filtration.
• Protein analysis: Reversed-phase-, and Size exclusion- HPLC (Agilent), LAL assay, Octet (titer assay)
• Spectroscopy: Nuclear Magnetic Resonance (NMR) (Varian 700), UV-Visible Spectroscopy, Fluorescence Spectroscopy (Anisotropy, FRET), Circular Dichroism (CD), Dynamic Light Scattering (DLS), Time Correlated Single Photon Counting (TCSPC), Isothermal Calorimetry (ITC), Small-angle X-ray Scattering (SAXS), Analytical Ultracentrifugation (AUC)
• Microscopy: Total Internal Reflection Fluorescence Microscopy (TIRF-M), Transmission Electron Microscopy (TEM), Atomic Force Microscopy (AFM)
• Productivity: Microsoft Word, Excel, and Power point, Origin, and SigmaPlot
• Programming: MATLAB, R, Python


Education

Year Degree Subject Institution
Year: 2010 Degree: Ph.D. Subject: Biochemistry Institution: CCMB, India
Year: 2003 Degree: M.Sc. Subject: Life Sciences Institution: JNU, India
Year: 2001 Degree: B.Sc. Subject: Agricultural Sciences Institution: BHU, India

Work History

Years Employer Title Department
Years: 2021 to Present Employer: Undisclosed Title: Head of Downstream Manufacturing Department:
Responsibilities:
Leads a team of downstream scientists to develop scalable downstream processes for viral vectors for use in Gene, Cell and Oncolytic therapies and for Vaccine production and for therapeutics proteins and antibodies.
Years Employer Title Department
Years: 2018 to 2021 Employer: Pall Corporation Title: Senior Purification Scientist Department:
Responsibilities:
Research
Years Employer Title Department
Years: 2016 to 2018 Employer: LakePharma, Inc. Title: Senior Scientist- Protein Sciences Department:
Responsibilities:
• Led team as a project manager
• Team lead-scale-up purification
• Led as a Study Director for a few projects with 10-15 team members
• Performed 150+ projects to their completion in one of the highest performing groups
• Worked in the downstream process development (DSP) such as purification of antibodies and mammalian cells at milligram to gram scales under low endotoxin condition
• Involved in purification and characterization of proteins from bacterial, yeast, insects and mammalian cells.
• Performed IMAC, IEX, affinity including GST, streptactin, softlink, ProA purifications, membrane enrichments using N2 bomb and sonication
• Performed SEC using S400, S200, S75 columns
• Performed large scale purification using BPG100 columns with 1-2 L of resins
• Characterized protein using aSEC, RP, CEX HPLC, DLS, CD
• Designed and executed refolding scouts to find optimal conditions for refolding
• Custom packed columns with bed volumes ranging from ml to L scale
• Performed ultrafiltration and diafiltration using TFF for up to 50 L scale
• Performed high through put protein purification
• Extensively worked on Akta Start, explorer, FPLC and Pilot
• Developed SOPs and prepared detailed report
• Trained and supervised direct report
• Trained and supervised new and old hires on the purification and characterization techniques including DLS, HPLC, TFF, custom resin packing, and Akta explorer
• Extensive client interactions to prepare statement of works, reports, and expand business opportunities in a one-to-one or team settings
• Handled multiple projects and client interactions simultaneously
• Collaborated with various teams for the completion of projects
• Directly involved in installation or training in new techniques such as DLS, Octet, and UPLC
Years Employer Title Department
Years: 2011 to 2016 Employer: University of Massachusetts Title: Postdoctoral Researcher Department:
Responsibilities:
• Investigated crosstalk between folding and aggregation of a model protein and its engineered variants
• Performed 15+ protein enrichments under minimal conditions to isotopically label protein
• Learned and performed 1D, 2D HSQC, 3D HNCO, HNCA, HNcoCA, HNcaCO, CBCAcoNH, and HNCACB experiments
• Processed NMR spectra and assigned protein under denatured condition
• Performed non-uniform sampling to reduce NMR time
• Developed a method using principal component analysis to investigate structural content of the unfolded state
• Developed skills for R, Python and MATLAB programming
• Performed protein folding experiments using CD, fluorescence measurements
• Identified that the folding information is present in the unfolded state
• Presented work in conferences and annual retreats
• Trained and supervised high school student and undergrads
Years Employer Title Department
Years: 2010 to 2011 Employer: Center for Cellular and Molecular Biology Title: Research Associate Department:
Responsibilities:
• Designed software for photo-acoustic spectroscopy (PAS) using LabVIEW
• Performed stability analysis on recombinant humanized monoclonal antibody, Bevacizumab (Avastin, Genentech/Roche) using reverse phase-HPLC (RP-HPLC)
Years Employer Title Department
Years: 2004 to 2010 Employer: Center for Cellular and Molecular Biology Title: Graduate Research Assistant Department:
Responsibilities:
• Studied misfolding and aggregation of mammalian prion protein
• Cloned, expressed and purified mammalian prion protein and its variants
• Discovered novel tertiary interactions between the N- and C-terminal regions of prion protein upon copper-binding using CD, fluorescence, SAXS, ITC, AUC, DLS, and NMR
• Identified loss of nucleation as a cause for inhibition of amyloid formation of UV-exposed prion protein using CD, fluorescence, AFM, and EM
• Authored three scientific papers and a book chapter
• Presented various professional seminars on prion diseases
• Trained and supervised high school students, undergrads and graduate students

Career Accomplishments

Awards / Recognition
HONORS AND AWARDS
• Best oral presentation award, in National Symposium on Biophysics-Biophysics in Medicine and Biology, organized by Indian Biophysical Society, India
• Young Scientist Bursary to attend 63rd Harden Conference – Protein folding and assembly in vitro and in vivo, Ambleside, UK, organized by Biochemical Society
• Senior Research Fellowship, Council of Scientific and Industrial Research (CSIR), India
• Junior Research Fellowship, CSIR, India

Additional Experience

Other Relevant Experience
ADDITIONAL INFO
• Workshop attended- Tips & Tricks: ACQUITY H-Class and QDa at Waters regional office
• Was point-of-contact with service engineers for several biophysical instruments such as ITC, CD, DLS, and time-resolved fluorometers
• Installed Endosafe Nexgen PTS reader with the remote help of application scientist

Fields of Expertise

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