Expert Details
Molecular Genetics and High-Complexity Diagnostics
ID: 738782
Florida, USA
MOLECULAR BIOLOGY:
• NGS (Next Generation Sequencing)
• Archer, RNAseq, Nextera, TruSeq
• Fragment Analysis
• PCR, Two-Step PCR, TouchDown-PCR, Nested PCR, Multiplex PCR, RT-PCR, qPCR
• Plasmid and BAC DNA isolation
• Recombineering, Cloning, CRISPR Design, Primer design
• Restriction enzyme digestions
• RNA isolation, Reverse transcription
CLINICAL LAB:
• Assay Development & Validation
• Knowledge of CAP requirements
• Illumina NGS platform (MiSeq & NextSeq)
• LightCyclers
• EZ1 BioRobots
• QiaSymphony
• Genetic Analyzers (3130XL & 3500XL)
• Cobas Ampliprep
ANIMAL:
• I.P. and I.V. injections in mice
• Mandibular and Ocular blood collection in mice
• Necropsy
• Colony maintenance
• Genotyping, Phenotyping
CELL BIOLOGY:
• Isolation of mononuclear cells from Peripheral blood, Spleen, Lymphnodes, Bone marrow and Bronchoalveolar lavages
• Flow Cytometry
Education
Year | Degree | Subject | Institution |
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Year: 2020 | Degree: MBA | Subject: Healthcare | Institution: Florida International University, Miami, FL |
Year: 2001 | Degree: MSc | Subject: Biotechnology & Medical Laboratory Sciences | Institution: University of Applied Sciences in Utrecht, The Netherlands |
Work History
Years | Employer | Title | Department |
---|---|---|---|
Years: 2018 to Present | Employer: Undisclosed | Title: Specialist - Molecular Genomics | Department: Molecular Genetics Laboratory |
Responsibilities:- Developing hospital wide precision care focused workflow optimization.- High complexity molecular testing – Developed dynamic Python scripted programs to make these tests low-complexity. - Successfully automated and streamlined various lab processes resulting in error reduction and time optimization. - Successfully set up and validated SNV, CNV and Fusion screening using NGS (Next Generation Sequencing) in solid tumors. - Setting up and validating PML/RARα by RT-PCR in patients with Acute Promyelocytic Leukemia. - MSI (microsatellite instability) testing using Fragment analysis to asses Lynch Syndrome. |
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Years | Employer | Title | Department |
Years: 2022 to Present | Employer: Undisclosed | Title: Founder | Department: |
Responsibilities:A LIMS (Laboratory Information Management System) provided as Custom designed Excel based worksheets that will aid in High-Complexity Laboratory testing. These worksheets will aid in decision support, automation, and sample custody and are capable of interfacing with several laboratory equipment. |
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Years | Employer | Title | Department |
Years: 2016 to 2018 | Employer: Northside Hospital | Title: Clinical Laboratory Scientist | Department: Molecular Laboratory |
Responsibilities:- Developing and Validating test to quantify mutations in MPL by qPCR.- Successfully Developed and Validated quantitation of CALR somatic mutation measurement to assess drug efficacy using fragment analysis. - Significantly improved Viral RNA extraction protocol for Zika testing. - Part of the Bone Marrow Transplant Group of Atlanta – Chimerism/STR Fragment Analysis to assess bone marrow engraftment using Genetic Analyzer and GeneMapper with <1% sensitivity. - Measuring viral titers (HIV, HCV, CMV and Zika) in clinical samples (Whole Blood, PBMCs, - Bone Marrow, Serum, Plasma, Urine and BAL) using AmpliPrep, QiaSymphony and IVD qRTPCR. |
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Years | Employer | Title | Department |
Years: 2011 to 2017 | Employer: Emory University / Stanford University | Title: Laboratory Manager | Department: Vaccine Center |
Responsibilities:- Organized the lab moving to Stanford University, including new lab design.- Management and genotyping of large mouse colony (>100 strains). - Successfully doubled amount of mouse strains while reducing total absolute cost and increased availability and volume of experimental mice. - Designing and creating new mouse strains using CRISPR. - Involved in coordinating Human and Non-Human Primate Clinical trials to learn prediction of effectiveness of vaccination. In charge of Biosafety and IACUC protocols. Involved in evaluation of personnel and hiring new personnel. Overseeing lab accounts to determine where additional savings can be applied. - Interacting with vendors and negotiating/obtaining quotes. |
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Years | Employer | Title | Department |
Years: 2004 to 2011 | Employer: Rockefeller University | Title: Laboratory Manager | Department: Innate Immunity |
Responsibilities:- Creating transgenic and knock-out/in mouse models using large DNA constructs and complex DNA cloning techniques.- Created an AICDA-miR155 Target mutant transgenic mouse model. Studying properties of cytidine deaminases during viral immune responses and in context of a variety of cancers. - Interacting with vendors and negotiating/obtaining quotes. Maintaining database of unique cell lines, microorganisms and mouse colony. - Webmaster. - Studying cytidine deaminases in the context of Multiple Myeloma. |
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Years | Employer | Title | Department |
Years: 2001 to 2003 | Employer: University of Miami School of Medicine | Title: Research Associate | Department: Diabetes Research Institute |
Responsibilities:- Monitoring immune status of clinical trial patients by flowcytometry and isolation of bone marrow cells from non-human primate vertebral bodies.- Studying properties of recombination systems in dsDNA viruses and E.coli. Developing an HSV-1 (Herpes Simplex Virus type 1) based portable recombineering system. - In charge of managerial duties. |
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Years | Employer | Title | Department |
Years: 2001 to 2001 | Employer: University of Pennsylvania Medical Center | Title: Research Associate | Department: |
Responsibilities:Performed a high-resolution genome screen of the murine genome to find loci that might play a role in susceptibility for MMTV (Mouse Mammary Tumor Virus). |
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Years | Employer | Title | Department |
Years: 1997 to 2000 | Employer: Utrecht University, School of Veterinary Medicine | Title: Microbiologist | Department: Wildlife and Laboratory animals |
Responsibilities:Trained new employees and students. Identified bacteria, yeasts and fungi by their biochemical characteristics and microscopy. Autopsies on different animals for laboratory animal screening and regular pathology. |
Career Accomplishments
Associations / Societies |
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ACHE – American College of Healthcare Executives (2019-Present) |
Licenses / Certifications |
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MB(ASCP)cm - 2016-Present Florida Supervisor in Molecular Pathology - 2018 Lean Six Sigma – Black Belt |
Awards / Recognition |
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Linxi Li Scolarship |
Publications and Patents Summary |
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12 publications |
Language Skills
Language | Proficiency |
---|---|
English | Fluent |
Dutch | Fluent |