Intellex Acquires Expert by Big Village

We're thrilled to announce that Intellex has acquired Expert by Big Village, effective March 22, 2024. This strategic move enhances our capabilities and strengthens our commitment to delivering exceptional solutions to our customers.

Stay tuned for more updates on how this acquisition will benefit our clients and experts.

For inquiries or more information, please contact us at info@intellex.com.

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Expert Details

Molecular Genetics and High-Complexity Diagnostics

ID: 738782 Florida, USA

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Expert has been working in Molecular Genetics for over 20 years and has extensive Research and Clinical experience. He hold a clinical license in Molecular Pathology in the state of Florida. His goal is to make healthcare advance through the use of precision care.

MOLECULAR BIOLOGY:
• NGS (Next Generation Sequencing)
• Archer, RNAseq, Nextera, TruSeq
• Fragment Analysis
• PCR, Two-Step PCR, TouchDown-PCR, Nested PCR, Multiplex PCR, RT-PCR, qPCR
• Plasmid and BAC DNA isolation
• Recombineering, Cloning, CRISPR Design, Primer design
• Restriction enzyme digestions
• RNA isolation, Reverse transcription

CLINICAL LAB:
• Assay Development & Validation
• Knowledge of CAP requirements
• Illumina NGS platform (MiSeq & NextSeq)
• LightCyclers
• EZ1 BioRobots
• QiaSymphony
• Genetic Analyzers (3130XL & 3500XL)
• Cobas Ampliprep

ANIMAL:
• I.P. and I.V. injections in mice
• Mandibular and Ocular blood collection in mice
• Necropsy
• Colony maintenance
• Genotyping, Phenotyping

CELL BIOLOGY:
• Isolation of mononuclear cells from Peripheral blood, Spleen, Lymphnodes, Bone marrow and Bronchoalveolar lavages
• Flow Cytometry

Education

Year Degree Subject Institution
Year: 2020 Degree: MBA Subject: Healthcare Institution: Florida International University, Miami, FL
Year: 2001 Degree: MSc Subject: Biotechnology & Medical Laboratory Sciences Institution: University of Applied Sciences in Utrecht, The Netherlands

Work History

Years Employer Title Department
Years: 2018 to Present Employer: Undisclosed Title: Specialist - Molecular Genomics Department: Molecular Genetics Laboratory
Responsibilities:
- Developing hospital wide precision care focused workflow optimization.
- High complexity molecular testing – Developed dynamic Python scripted programs to make these tests low-complexity.
- Successfully automated and streamlined various lab processes resulting in error reduction and time optimization.
- Successfully set up and validated SNV, CNV and Fusion screening using NGS (Next Generation Sequencing) in solid tumors.
- Setting up and validating PML/RARα by RT-PCR in patients with Acute Promyelocytic Leukemia.
- MSI (microsatellite instability) testing using Fragment analysis to asses Lynch Syndrome.
Years Employer Title Department
Years: 2022 to Present Employer: Undisclosed Title: Founder Department:
Responsibilities:
A LIMS (Laboratory Information Management System) provided as Custom designed Excel based worksheets that will aid in High-Complexity Laboratory testing. These worksheets will aid in decision support, automation, and sample custody and are capable of interfacing with several laboratory equipment.
Years Employer Title Department
Years: 2016 to 2018 Employer: Northside Hospital Title: Clinical Laboratory Scientist Department: Molecular Laboratory
Responsibilities:
- Developing and Validating test to quantify mutations in MPL by qPCR.
- Successfully Developed and Validated quantitation of CALR somatic mutation measurement to assess drug efficacy using fragment analysis.
- Significantly improved Viral RNA extraction protocol for Zika testing.
- Part of the Bone Marrow Transplant Group of Atlanta – Chimerism/STR Fragment Analysis to assess bone marrow engraftment using Genetic Analyzer and GeneMapper with <1% sensitivity.
- Measuring viral titers (HIV, HCV, CMV and Zika) in clinical samples (Whole Blood, PBMCs,
- Bone Marrow, Serum, Plasma, Urine and BAL) using AmpliPrep, QiaSymphony and IVD qRTPCR.
Years Employer Title Department
Years: 2011 to 2017 Employer: Emory University / Stanford University Title: Laboratory Manager Department: Vaccine Center
Responsibilities:
- Organized the lab moving to Stanford University, including new lab design.
- Management and genotyping of large mouse colony (>100 strains).
- Successfully doubled amount of mouse strains while reducing total absolute cost and increased availability and volume of experimental mice.
- Designing and creating new mouse strains using CRISPR.
- Involved in coordinating Human and Non-Human Primate Clinical trials to learn prediction of effectiveness of vaccination. In charge of Biosafety and IACUC protocols. Involved in evaluation of personnel and hiring new personnel. Overseeing lab accounts to determine where additional savings can be applied.
- Interacting with vendors and negotiating/obtaining quotes.
Years Employer Title Department
Years: 2004 to 2011 Employer: Rockefeller University Title: Laboratory Manager Department: Innate Immunity
Responsibilities:
- Creating transgenic and knock-out/in mouse models using large DNA constructs and complex DNA cloning techniques.
- Created an AICDA-miR155 Target mutant transgenic mouse model. Studying properties of cytidine deaminases during viral immune responses and in context of a variety of cancers.
- Interacting with vendors and negotiating/obtaining quotes. Maintaining database of unique cell lines, microorganisms and mouse colony.
- Webmaster.
- Studying cytidine deaminases in the context of Multiple Myeloma.
Years Employer Title Department
Years: 2001 to 2003 Employer: University of Miami School of Medicine Title: Research Associate Department: Diabetes Research Institute
Responsibilities:
- Monitoring immune status of clinical trial patients by flowcytometry and isolation of bone marrow cells from non-human primate vertebral bodies.
- Studying properties of recombination systems in dsDNA viruses and E.coli. Developing an HSV-1 (Herpes Simplex Virus type 1) based portable recombineering system.
- In charge of managerial duties.
Years Employer Title Department
Years: 2001 to 2001 Employer: University of Pennsylvania Medical Center Title: Research Associate Department:
Responsibilities:
Performed a high-resolution genome screen of the murine genome to find loci that might play a role in susceptibility for MMTV (Mouse Mammary Tumor Virus).
Years Employer Title Department
Years: 1997 to 2000 Employer: Utrecht University, School of Veterinary Medicine Title: Microbiologist Department: Wildlife and Laboratory animals
Responsibilities:
Trained new employees and students. Identified bacteria, yeasts and fungi by their biochemical characteristics and microscopy. Autopsies on different animals for laboratory animal screening and regular pathology.

Career Accomplishments

Associations / Societies
ACHE – American College of Healthcare Executives (2019-Present)
Licenses / Certifications
MB(ASCP)cm - 2016-Present
Florida Supervisor in Molecular Pathology - 2018
Lean Six Sigma – Black Belt
Awards / Recognition
Linxi Li Scolarship
Publications and Patents Summary
12 publications

Language Skills

Language Proficiency
English Fluent
Dutch Fluent

Fields of Expertise

clinical genetics, genetics, genetics, vaccines, diseases and health, applied molecular biology, human genetics, medical genetics, molecular genetics, molecular genetics testing, molecular genetic pathology

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