Request Expert

Experthas developed and participated in assay development and troubleshooting for variety of target molecules with special skill in development of ELISA on different platforms (spectroscopy, luminescence and electrochemical). He has also acquired very good grasp on nanoparticles based rapid assays. The target molecules for the assays varied from small organic molecules, carbohydrates, proteins, and cells.

He has recently evolved into development of molecular biology assays. He has a sharp analytical mind and identifies the bugs very early in the stage of development

troubleshooting services in all aspects of biotechnology

Analytical research scientist with over 9 years of experience in development of immunoassays and molecular biology assays from scratch. My work includes assay design, vendor selection, and assay development with steps including reagent development, assay optimization, troubleshooting to make the product compliant to regulatory requirements. Experience managing multiple assay development, laboratory bench studies, writing scientific reports and maintaining research lab operations and equipment.

WORK EXPERIENCE:

1. Assay development
 Developed lateral flow assay for PCR based detection of tuberculosis.
 Developed lateral flow assay for PCR based detection of typhoid.
 Developed lateral flow immunoassay for bacterial cell detection (typhoid).
 Developed lateral flow assay for detection of antibodies for detection if different analytes (typhoid, malaria, dengue, Luteinizing hormone, progesterone).
 Developed LAMP assay for tuberculosis detection.
 Developed enzymatic detection system for Glycosylated hemoglobin (HbA1c).
 Developed ELISA based assay for detection of environmental contaminants.
 Developed ELISA based assay for detection of carbohydrates in bacterial infection.
 Developed ELISA based assay for detection of HbA1c.
 Developed ELISA based assay for detection of Vitamin D3.
 Developed electrochemical assay based high sensitivity concept system for detection of
different analytes.

2. Bioconjugation
 Antibody conjugation with drug molecules (ADC) such as taxol and sainomycin.
 Antibody conjugation with fluorescent molecules such as quantum dots, FITC and rhodopsin.
 Antibody as well BSA conjugation with nanoparticles of gold, silver, core-shell, palladium, and carbon nanotubes.
 Conjugation of proteins with peptides, haptens to inject in laboratory animals.
 Conjugation of nanoparticles with small molecules, peptides, carbohydrates for assay development.

3. Nanoparticles synthesis
 Metal nanoparticles synthesis (Gold, silver, palladium, iron, core-shell) using wet chemical
method.
 Carbon nanotubes synthesis by CVD and modification by wet chemical methods.
 Quantum dots preparation by chemical route.

4. Pharmacovigilance
 Literature Triage.
 Case Analysis
 Case Review
 Drug safety report.