Intellex Acquires Expert by Big Village

We're thrilled to announce that Intellex has acquired Expert by Big Village, effective March 22, 2024. This strategic move enhances our capabilities and strengthens our commitment to delivering exceptional solutions to our customers.

Stay tuned for more updates on how this acquisition will benefit our clients and experts.

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Expert Details

Biological Staining, Fluorescent Probes, Selective Uptake into Live Cells

ID: 723113 United Kingdom

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Expert has a wide-ranging knowledge of, and practical experience with, dyes used in biomedicine – many of which were originally used industrially, eg as textile or food dyes. He has produced novel biological staining applications making use of such dyes; devised analytical procedures for many of them; and prepared trouble-shooting schemes for those using more widely applied dyes.

Novel staining procedures – Expert has shown that new staining procedures, often simpler and more reliable, can be dveloped. For instance those using fuchsine, with fixed tissues. More recently he has developed expertise in assessing which dye structures will result in particular intravellular localization within living cells. The underlying models have also proved applicable to xenobiotics in general, such as drugs, herbicides and pollutants.

Analytical procedure – He has devised widely applicable methods, which for instance can be applied to most acid (ie anionic) and basic (ie cation), mordant (ie dye lakes and metallized), neutral and solvent dyes in general use; as well as to many direct dyes and fluorescent brightening agents.

Trouble shooting – Schemes to trouble-shoot many histopathological stains have been clarified.

Cell biology – involves an integrated understanding of the structure (anatomy) and function of biological cells. Expert has considerable experience with interpreting images of a wide variety of cell types [animal, plant and microbial] using a range of microscopic methods and staining reagents. This has proved valuable to specialists first involved with applying a variety of xenobiotics to biological specimens, and using microscopy to assess outcomes.

By inspecting the chemical structures of a set of novel fluorochromes, & estimating their intracellular localization patterns using QSAR models, he could recommend which were most suitable as cell and molecular biological fluorescent probes. He suggested colored labels for young of marine animals of commercial significance, to assess reproductive efficacy under various circumstances.He devised an in-house trouble-shooting guide for a laboratory engaged in the biological staining of a specific type of animal biopsy. He inspected chemical structures of proposed novel pharmaceutical agents, and provided predictions [based on QSAR models] of where they would localise in the appropriate cell type, thus permitting synthesis of the more promising structures. He provided analytical protocols and criteria to permit a biological stain vendor to decide if certain dyes available to him would be of suitable quality for use as biological stains.


Year Degree Subject Institution
Year: 1961 Degree: BSc Subject: Chemistry Institution: University of Sheffield
Year: 1964 Degree: PhD Subject: Physical organic chemistry Institution: University of Sheffield

Work History

Years Employer Title Department
Years: 2000 to Present Employer: Undisclosed Title: Honorary Senior Research Fellow Department: Div of Integrated Biology
He carries out biomedical research.
Years Employer Title Department
Years: 1989 to 1996 Employer: University of Sheffield Title: Reader Department: Dept of Biomedical Science
He engaged in research, teaching and administration within the Department; also helped run an in-house consultancy "Datascope Services".

International Experience

Years Country / Region Summary
Years: 2006 to Present Country / Region: USA Summary: He is advising which dyes might be most usefully synthesised for labeling a specific biological target.
Years: 2001 to 2001 Country / Region: Germany Summary: He devised a trouble-shooting guide for a reagent supplier, for circulation amongst their customers.

Career Accomplishments

Associations / Societies
Biological Stain Commission
National Society of Histotechnology
Royal Microscopic Society
Scottish Microscopy Group
Publications and Patents Summary
He has more than 150 peer reviewed research papers, 4 major books, and a wide variety of minor publications --- mostly in the field of interaction of dyes and fluorochromes with biological specimens alive and dead.

Additional Experience

Training / Seminars
Recently he has run short courses concerning with predicting the intracellular localisation of xenobiotics [eg drugs, dyes, herbicides] at the New University of Ireland (Galway) and North Eastern (USA). When employed by the University of Sheffield he organised and taught on a variety of undergraduate & graduate school courses in biomedicine.
Other Relevant Experience
Predicting the uptake and intracellular localisation of small-molecule xenobiotics [eg drugs, dyes, fluorochromes, herbicides] in living cells [human, animal, plant, microbial] from a knowledge of the xenobiotics chemical structure.

Fields of Expertise

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