Expert Details

Recombinant Protein Expression, Purification, Cell-Based Assay Systems

ID: 722883 Virginia, USA

Expert has expertise is in understanding the basic concepts of receptor-ligand interactions. He notes that this is one of the most fundamental processes present in creatures, from simple “cell-free organisms” such as viruses to complex multiorgan organisms like humans. As most of these interactions involve proteins, Expert has expertise in the biochemistry of proteins involved in such processes. With the availability of the genome sequence of a large number of human pathogens as well as humans, he has worked on exploiting bioinformatical tools for gene selection, cloning, sequencing, and their recombinant expression and purification, utilizing a wide variety of chromatography techniques. He has developed protocols for purification of proteins from inclusion bodies, cytoplasm, periplasm, and culture media. Expert notes that these purified proteins are subsequently characterized for their interactions with other proteins and carbohydrate-based receptors.

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Expert has served as an expert for several funding agencies in the U.S. and abroad for evaluation of grant applications requiring expertise on host-pathogen interactions.He serves as a troubleshooter on protein expression and purification problems with several researchers within and outside his institute.

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Education

Year	Degree	Subject	Institution
Year: 1997	Degree: Ph.D.	Subject: Disease Mechanisms and Control	Institution: National Institute of Immunology
Year: 1992	Degree: M.Sc.	Subject: Biotechnology	Institution: Banaras Hindu University
Year: 1990	Degree: B.Sc.	Subject: Zoology	Institution: University of Delhi

Work History

Years	Employer	Title	Department
Years: 2003 to Present	Employer: Undisclosed	Title: Research Assistant Professor	Department:
Responsibilities: Expert runs a laboratory that investigates disease pathogenesis across phylogenetic lines, with major focus on malaria. Specifically, he investigates the molecular aspects of host-pathogen interactions, with the eventual goal of designing novel intervention strategies that can be employed to block these interactions.
Years	Employer	Title	Department
Years: 1997 to 2003	Employer: National Institutes of Health	Title: Research Fellow	Department:
Responsibilities: Available upon request.

Government Experience

Years	Agency	Role	Description
Years: 1997 to 2003	Agency: National Institutes of Health	Role: Research Fellow	Description:

Career Accomplishments

Associations / Societies
Expert is a member of the American Association for Advancement of Science and the American Society for Tropical Medicine and Hygiene.

Professional Appointments
He is Associate Editor of Infection, Genetics & Evolution.

Awards / Recognition
Expert received the Keystone Scholarship to attend the “Malaria's Challenge: From Infants to Genomics to Vaccines” meeting (2002). He received the Fellows Award for Research Excellence by the National Institutes of Health, USA (2001); the Young Scientist Medal of Indian National Science Academy (INSA), New Delhi, India (1999); the Travel Award by Council of Scientific & Industrial Research to attend American Society for Cell Biology meeting (1996); the Young Scientist award at the Fourth International Symposium on Eukaryotic Cell Surface Macromolecules, New Delhi, India (1996); the Council of Scientific & Industrial Research Fellowship for Doctoral Studies (1992-1997); and, the Gold Medal for obtaining first position in the order of merit in M.Sc. Biotechnology examinations of Banaras Hindu University, India (1992).

Awards / Recognition

Expert received the Keystone Scholarship to attend the “Malaria's Challenge: From Infants to Genomics to Vaccines” meeting (2002). He received the Fellows Award for Research Excellence by the National Institutes of Health, USA (2001); the Young Scientist Medal of Indian National Science Academy (INSA), New Delhi, India (1999); the Travel Award by Council of Scientific & Industrial Research to attend American Society for Cell Biology meeting (1996); the Young Scientist award at the Fourth International Symposium on Eukaryotic Cell Surface Macromolecules, New Delhi, India (1996); the Council of Scientific & Industrial Research Fellowship for Doctoral Studies (1992-1997); and, the Gold Medal for obtaining first position in the order of merit in M.Sc. Biotechnology examinations of Banaras Hindu University, India (1992).

Publications and Patents Summary
He has authored over 30 publications in biochemistry and molecular biology journals.

Additional Experience

Training / Seminars
Expert has served as the chief instructor for a bioinformatics course at the Foundation for Advanced Education in Sciences, the Graduate School at the National Institutes of Health, Bethesda, MD.

Vendor Selection
He has expertise in laboratory design and workflow with respect to a typical biochemistry, molecular biology, and cell culture laboratory and has a vast knowledge about the best sources for the equipment required for such work.

Language Skills

Language	Proficiency
Hindi	Expert is fluent in Hindi.

Fields of Expertise