Expert Details
Organic Chemistry, Analytical Chemistry, Biochemistry, Cell Biology, Photochemistry, Chromatography
ID: 730236
New York, USA
Extensive experience in developing immunoassays and in raising and screening polyclonal and monoclonal antibodies. Antibody production has included antibodies raised against proteins and low molecular mass molecules (less than 0.5 kD). Extensive experience in customization of immunoassays, including enzyme linked immunosorbent assays (ELISA), immunoblotting (Western blots), immunoprecipitations, and affinity column chromatography. Using these approaches the expert has been able to detect in vitro and in situ the DNA lesion, 8-oxoguanine.
Extensive experience in the identification of molecular targets for naturally occurring toxins through combinations of mass spectral and proteomic analyses. Using LC/MS/MS the expert has been able to detect and quantitate DNA damage, as well as alterations in metabolic pathways, in small mammals exposed to toxins. In addition, she has developed assays by which immobilized toxin is used to pull down potential protein targets in tissue homogenates. Identification of protein targets is performed by combining biochemical, immunochemical and proteomic analyses. The expert also has detected and quantitated toxins from animal isolates and dietary supplements.
Education
Year | Degree | Subject | Institution |
---|---|---|---|
Year: 2001 | Degree: Postdoctoral | Subject: Cell Biology/Biochemistry | Institution: SUNY at Stony Brook |
Year: 1995 | Degree: Ph.D. | Subject: Organic Chemistry | Institution: University of Connecticut |
Year: 1992 | Degree: M.S. | Subject: Organic Chemistry | Institution: University of Connecticut |
Year: 1988 | Degree: B.S. | Subject: Chemistry | Institution: University of Connecticut |
Work History
Years | Employer | Title | Department |
---|---|---|---|
Years: 2001 to 2011 | Employer: SUNY at Stony Brook | Title: Research Scientist | Department: Pharmacology |
Responsibilities:Available upon request. |
|||
Years | Employer | Title | Department |
Years: 1998 to 2011 | Employer: SUNY at Stony Brook | Title: Research Instructor | Department: Pharmacology |
Responsibilities:Available upon request. |
|||
Years | Employer | Title | Department |
Years: 1995 to 2001 | Employer: SUNY at Stony Brook | Title: Postdoctoral Research Associate | Department: Pharmacology |
Responsibilities:Trained in cell biology/biochemistry. |
|||
Years | Employer | Title | Department |
Years: 1995 to 2001 | Employer: | Title: | Department: |
Responsibilities:Available upon request. |
Career Accomplishments
Associations / Societies |
---|
American Chemical Society, American Society for Cell Biology. Chemical Consultants Network |
Publications and Patents Summary |
---|
The expert has published in peer reviewed journals and has four invention disclosures. |
Fields of Expertise
organic photochemistry, protein modification, protein-protein crosslinking, immunoassay, monoclonal antibody production, polyclonal antibody, monoclonal antibody, high-performance liquid chromatography/mass spectroscopy, high-performance liquid chromatography, oxidative stress, flash chromatography, phase-contrast microscopy, protein purification method, electron transfer, immunocytochemistry, thin-layer chromatography, electrospray ionization, fluorescence microscopy, chromatography, affinity chromatography