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For over 25 years, Expert has been actively engaged in biochemical research either at the bench or in supervising a research group of about 10 technicians, students, and post-doctoral fellows. His biochemical expertise deals with carbohydrates, lipids, proteins, glycoproteins, and polysaccharides. In addition, he is proficient in a variety of modern molecular biology approaches to address biochemical and cell biological questions. Most of the 95 peer-reviewed papers which he has published are in biochemical journals.

Expert has been directing a research program characterizing two liver specific membrane receptors involved in receptor mediated endocytosis and the clearance of several different carbohydrate-containing molecules. He has extensive experience characterizing the specificity and affinity of receptor interactions and subsequent internalization of receptor-ligand complexes. He is also familiar with characterizing the subunit composition of receptors as well as their purification, characterization, and cloning at the molecular level. Additionally, he is knowledgeable of site-directed mutagenesis approaches to studying the structure-function of receptors reconstituted in transfected cells.

These areas are essentially identical and represent expertise that Expert has developed and strengthened in his laboratory research after his doctoral work. Many of his publications can be classified in this area. He has regularly attended and presented his research at the annual national meetings of the American Society for Cell Biology. Expert's laboratory has used a wide range of techniques in this area. These include electron microscopy, fluorescence microscopy, tissue (cell) culture, and liver cell preparation.

Expert is an established expert in the field of receptor mediated endocytosis and has been publishing actively on this process as well as receptor characterization since 1980. He has had continuous NIH supported funding since 1979 for projects investigating the mechanisms controlling receptor mediated endocytosis and receptor recycling. A high percentage of his publications have dealt with these topics.

A significant portion of Expert's research has been devoted to biochemistry and the synthesis and degradation of hyaluronic acid. His group was the first in the world to clone the gene for the enzyme that allows Group A streptococcal bacteria to synthesize hyaluronic acid. They also have experience in studies dealing with the interaction between hyaluronic acid and fibrinogen and its effect on blood clotting, and they are studying other hyaluronic acid binding proteins and receptors that are physiologically significant. For example, the group is purifying and cloning a liver specific receptor that removes circulating hyaluronic acid from the blood. They also have expertise with unique chemical modifications of hyaluronic acid that allow them to make unusual and extremely useful probes to study its metabolism and interactions with cells.

A small fraction of Expert's research effort and publications over the last 10 years has dealt with cell surface interactions. This has involved the preparation of chemically defined cell culture substrata and the examination of the mechanism by which cells can adhere to and spread on them.

Expert is presently a consultant for a new biotechnology company.He has been a Teltech consultant since 1986 and has participated in more than 40 client events.